Fig. 2.

Inheritance of stress-induced transcriptional activation of LUC. (A) Crossing scheme for the recreation of the ddm1-2 mutant line with the naïve LUC transgene. P0: ddm1-2 was crossed with WT LUC25, F1: heterozygous for ddm1 and carrying the hemizygous naïve LUC transgene, F2: ddm1 homozygous mutants are segregated in the progeny. F2 seedlings were separated into two subpopulations, one of which was subjected to heat stress. Bioluminescence images were captured (B), and each plant was genotyped at the DDM1 and LUC loci. (B) Bioluminescence images of segregating progeny of a hybrid between ddm1-2 and LUC25 (A). The F2 seedlings were expected to include 18.75% of individuals homozygous for the ddm1-2 mutation and carrying the LUC transgene, these were predicted to display enhanced luminescence. Rows of LUC25 and mom1 LUC25 plants are shown as a control. Note, LUC signals in mom1 after heat stress are restricted to roots. (C) Bioluminescence images of segregating progeny of a cross between ddm1-2 mom1 LUC25 (for details, see Fig. S3). The F2 progeny is expected to include 4.69% of individuals homozygous for both ddm1-2 and mom1 and carrying the LUC transgene. White arrowheads point toward ddm1-2 mom1 double mutant plants, as revealed by the genotyping of the population at DDM1 and MOM1 loci. Quantification of LUC signals is displayed in Fig. S4. (D) Bioluminescence images of ddm1 LUC25 and ddm1 mom1 LUC25 F3 progenies (as depicted in A) of F2 heat stressed parents at the seedlings stage (+stress) or nonstressed controls (−stress). Two independent F3 populations (upper or lower row) derived from two ddm1 LUC25 or ddm1 mom1 LUC25 F2 plants (B and C).