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. 2014 May 27;111(23):8434–8439. doi: 10.1073/pnas.1407849111

Fig. 2.

Fig. 2.

The Ub Y59-E51 loop. (A) The effects of Ub Y59 substitutions on di-Ub synthesis by Cdc34. (B) Overlay of 1H-15N HSQC spectra for wild-type Ub (black contours) and Y59F Ub (magenta contours). Amides located in the K48-Y59 loop of Ub are labeled. Arrows indicate where the amide resonances for the wild-type Ub K48-Y59 loop have shifted to in the Y59F spectrum. (C) Histogram of amide chemical shift perturbations between wild-type and Y59F Ub. The amide chemical shift changes for each residue in wild-type and Y59F Ub were determined using the spectra shown in B and the equation ΣΔδ = |(Δδ1H)| + |(0.2) × (Δδ15N)|.