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. 2014 May 27;111(23):8565–8570. doi: 10.1073/pnas.1405514111

Fig. 2.

Fig. 2.

Soluble IL-15 component released is not sufficient for IL-15 action: generation of an uncleavable form of IL-15Rα. (A) Flow cytometric analyses of intracellular IL-15 expression (MFI) within Kit225 and NK92 cells following 24 h coculture separated from wt.ILR or wt.IL-15Rα HEK-293 cells by an insert (white bars) or not (black bars). (B) Flow cytometric analysis of the percentage of p-Stat5 positive Kit225 cells following 1 h coculture with wt.ILR HEK-293 cells separated by an insert (white bar) or not (black bar). (C) Flow cytometric analysis of p-Stat5 expression (MFI) within Kit225 cells following 1 h stimulation with 10 pM and 500 pM of sILR. (D) ELISA detection of sIL-15Rα released from the different annotated point-mutated IL-15Rα HEK-293 transfected cells after 24 h of culture. Dashed line indicates the ELISA detection threshold (1 pM). (E) ELISA measurement of sILR released in the culture supernatant from wt.ILR, uc.ILR, and wt.IL-15Rα HEK-293 transfected cells after 24 h of culture. Dashed line indicates the ELISA detection threshold (2 pM). All data are representative of at least three separate experiments. ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001.