Fig. 4.

Concomitant deletion of VEGF does not impact HIF1-PPN–induced bone accrual. (A–C) Histological sections of tibiae. Boxed regions are shown in higher magnification in the lower panels. (D–F) Evaluation of bone vasculature by Microfil casting and µCT. The avascular growth plate is at the top of each cast. Note the dense accumulation of small-caliber vessels in SH-dox mice compared with control or SHΔV-dox. (G) Quantification of bone volume per total volume of tibia cancellous bone via µCT. There was no difference between SH-dox and SHΔV-dox mice. (H) Quantification of marrow fibrosis. Fibrotic marrow area normalized to total marrow area on three H&E sections per mouse. *P < 0.05, n = 4 (control); n = 4 (SH-dox); n = 3 (SHΔV-dox). Control mice were sex-matched littermates without SP7-tTA, subjected to same dox regime as experimental groups.