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. 2014 Jun 17;8:65. doi: 10.3389/fncir.2014.00065

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Copyright © 2014 Yassin, Radtke-Schuller, Asraf, Grothe, Hershfinkel, Forsythe and Kopp-Scheinpflug.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NO-mediated suppression of KCC2 requires intracellular cGMP and Zinc. (A) KCC2 activity was monitored with the pH-sensitive dye BCECF in SHSY-5Y cells that express endogenous KCC2. Cells were incubated for 5 min with K⁺ free extracellular solution, and the rate of intracellular pH change following application of NH₄Cl (5 mM) was monitored (see Materials and Methods). (A,B) Plots of NH⁺₄- mediated acidification rates (mean ± s.e.m.) in control, treated with NO, NO+ODQ, NO+TPEN, or ODQ+TPEN+furosemide. Significance was assessed using a One-Way ANOVA. Ns are given in the bars. ^***p ≤ 0.001.