Fig. 6.

Western blot analysis of fracture callus active beta catenin levels. Representative western blots for activated and total β-catenin protein levels in fracture callus lysates at Day 9 post-fracture. Data are presented as the densitometric ratio of activated/total β-catenin. Alcohol causes a significant decrease in the ratio of active (hypophosphorylated) β-catenin when compared with saline-exposed mice. LiCl treatment caused a 52% increase in activated β-catenin compared with alcohol treatment alone. Groups not sharing a letter are statistically significant, P ≤ 0.05 using one-way ANOVA and Tukey's multiple comparison procedure. N = 4–8 mice/treatment group.