Figure 3.
Influence of initial seeding cell densities on the microglia culture. Mixed retinal cells seeded at 0.75–1 × 106 cells/cm2 produced the highest cell numbers. (a) 1 × 106 cells/mL. (b) 0.75 × 106 cells/mL. (c) 0.5 × 106 cells/mL. (d) 0.25 × 106 cells/mL. (e) 0.13 × 106 cells/mL. After 15–20 days of culture, retinal microglial cells were observed using a phase-contrast Olympus microscope and Prog Res C14 imaging software. Magnification ×20. Cells were counted using haemocytometer and trypan blue was used to distinguish viable and dead cells. Error bars are SEM. *P < 0.05, compared to others groups.