Figure 4.

Microglial cells isolated from the mixed culture on different days influence the microglial cell numbers and morphology. (a) Removing nonadherent cells on day 5 yielded the least number of cells. ((b) and (c)) Microglial cultures on day 10. ((d) and (e)) Microglial cultures on day 15. Day 10 seems to be ideal time to wash away loosely adherent cells and dead cells. Day 15 seems to affect the morphology of the cell. Microglial cells were observed using a phase-contrast Olympus microscope and Prog Res C14 imaging software. Magnification ×20. (f) Microglial cells were counted using haemocytometer after different days of the initial seeding. Error bars are SEM for 2 replicate cultures. *P < 0.05 compared to day 5 and day 15.