FIG. 4.

rSCC-61 cells are associated with mesenchymal-to-epithelial transition (MET). (A) Semi-quantitative PCR and Western blot analysis of Vimentin and E-cadherin. The relative expression of vimentin and E-cadherin in SCC-61 and rSCC-61 was determined using semi-quantitative PCR (n=1). 18S RNA amplification was used as endogenous control. Three out of five biological replicates are shown for the Western blot analysis; quantification is based on all five replicates and taking into account the differences in GAPDH (Vimentin: ***p<0.001, n=5; E-cadherin: **p=0.01, n=5). (B) Table showing the protein ratio of integrin isoforms identified by the SILAC proteomics analysis. (C) Trans-well migration assay. The migration properties of SCC-61 and rSCC-61 cells were quantified using the trans-well migration assay described in the “Supplementary Materials and Methods” section (***p<0.001, n=3).