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. 2014 Jun 9;14:100. doi: 10.1186/1471-2466-14-100

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Copyright © 2014 Balloy et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Representative western blots of three sputum extracts containing low (n°36), medium (n°25) and high (n°9) flagellin concentrations. Four concentrations of protein (1, 2.5, 5 and 10 μg/well) from 3 different expectorations and standard curve of flagellin (0.1, 0.25, 0.5, and 1.0 ng/well) were loaded on 10% acrylamide gel and fractionated by SDS-PAGE (see Methods). Note i) that the recombinant non glycosylated flagellin (right hand side) has a molecular weight lower (40 kD) than the native flagellin (left hand side) present in the sputum (49 kD) and corresponding to flagellin type b, and 2) the presence a faint band with a 40 kD mw observed for patients 25 and 9, and corresponding to flagellin type a.