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. 2014 Jun 9;11:109. doi: 10.1186/1743-422X-11-109

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Copyright © 2014 Matthaei et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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RHDV and RHDVa replication, excretion and tissue distribution in rabbit kittens infected with a high dose of inoculum. Shown are virus genome copy numbers per μl whole blood (A, B) or rectal swab (C, D) measured via RT-qPCR in samples taken from RHDV (A, C) and RHDVa (B, D) infected kittens 4–5 weeks of age at the indicated time points. SDL indicates the genome concentration relative to 20 genome copies detected in the RT-qPCR, which is considered the safe detection limit. E: Virus loads in tissues of RHDV and RHDVa infected kittens, 5 dpi. The safe detection limit of our assay in these tissues was 40 genome copies/mg on average (range: 35–71 depending on the amount of tissue the RNA was extracted from) or 12 genome copies/μl bile.