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A flow diagram outlining the experimental design of samples containing fox faeces with a known number of Echinococcus multilocularis eggs (α2 and β2). 144 samples were divided into two batches for DNA extraction at two different laboratories. Each of the laboratories then carried out two of the four PCR methods on the DNA extractions done at their laboratory (solid line) as well as on DNA extractions sent from the other laboratory (dotted line).
