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. 2014 May 28;7:246. doi: 10.1186/1756-3305-7-246

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Copyright © 2014 Øines et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Depicting results from batches α2 egg sieving and β2 DNA-fishing ordered by number of eggs (shown with the increasing intensity of the blue gradient from zero eggs to 500+ eggs). Individual replicate results are marked either as negative (red) or positive (green) after being analysed using the multiplex PCR (mPCR), Taq-PCR, EVA-PCR and CO1rtPCR. Batch α2 is on the left side of the blue gradient whilst β2 is on the right side of the central blue column. Any greyed positions indicate replicates which were not available due to lack of DNA.