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. 2014 May 28;7:246. doi: 10.1186/1756-3305-7-246

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Copyright © 2014 Øines et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Results from analyses of results from batches α1 and β1, eggs only. The Y-axis shows the percentage of E. multilocularis egg spiked samples that were detected by each of four PCR methods (Taq-PCR blue diamond; EVA-PCR green square; mPCR red triangle; and CO1rtPCR purple circle), after using two different DNA extraction methods – egg sieving (dotted line, N = 24) and DNA-fishing (solid line, N = 24). The 95% confidence interval is shown for the detection level and given that all overlap; no significant differences were seen between the different methods with samples containing eggs only, with no inhibitors. The CO1rtPCR was run on α1 samples only.