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. 2014 May 28;7:246. doi: 10.1186/1756-3305-7-246

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Copyright © 2014 Øines et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Graph showing the percentage of E. multilocularis egg spiked faecal samples that were detected by each of four PCR methods (Taq-PCR blue diamond; EVA-PCR green square; mPCR red triangle; and CO1rtPCR purple circle) after using two different DNA extraction methods – egg sieving (dotted line, N = 59) and DNA-fishing (solid line, N = 59), once the negative and the high positive control samples (groups E, n = 12, and F, n = 1) were excluded from the data set. The 95% confidence interval is given for the detection level.