Table 1.

Results of the molecular analysis of E. multilocularis eggs-only samples using two extraction methods

Method	Egg sieving DNA extraction		DNA-fishing extraction
	No. +ve/no. examined	Percentage detected [95% CI]	No. +ve/no. examined	Percentage detected [95% CI]
Taq-PCR	24/24	100% [86.2-100]	22/24	91.7% [74.2-97.7]
EVA-PCR	21/24	87.5% [70.0-95.7]	21/24	87.5% [68.9-95.7]
mPCR	24/24	100% [86.2-100]	20/24	83.3% [64.1-93.3]
CO1rtPCR	23/24	95.8% [79.7-99.2]	Not examined

A summary of the results of the molecular analysis of pure E. multilocularis egg samples using two different DNA extraction methods (egg sieving and DNA-fishing) and subsequent analysis with up to four different PCR methods (Taq-PCR, EVA-PCR, mPCR and CO1rtPCR. Three replicates were run for all methods except for EVA-PCR which was run only once due to limited material. A sample was categorised as being positive if either of the replicates produced a positive result. Egg numbers added to each sample are available in Additional file 1: Table S1.