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. Author manuscript; available in PMC: 2014 Jun 17.
Published in final edited form as: J Immunol. 2013 Jan 18;190(4):1510–1518. doi: 10.4049/jimmunol.1201017

Figure 5.

Figure 5

IL-27 inhibits the activation of caspases. CFSE-labeled WT naïve CD4+ T cells were stimulated as in Figure 2 in the presence or absence of rIL-27. Anti-FasL blocking antibody was added where indicated either on day 0 (top row) or day 2 (bottom row). Numbers indicate the percentages in the respective gates for 7-AAD+ or 7-AAD− cells. (B) Intracellular staining for activated caspase 3 in cells stimulated for 2 or 3 days, in the absence or presence of rIL-27, and/or anti-FasL antibody added on day 0. Broken lines represent negative control stained with an isotype control antibody. (C) Staining for activated caspase 8 and 7-AAD in cells stimulated for 3 days, in the absence or presence of rIL-27, and/or anti-FasL antibody on day 0. Numbers in dot plots represent the percentage cells in respective gates to total cells. Data are representative from one of at least two experiments.