Figure 2.

Compound 8f induces histone acetylation in MEF cells and apoptosis in T cells. (a,b) MEF cells were treated with 8f or VPA for 6 h and then lysed for Western blot analysis to detect the acetylation level of histone H3 and H4. Quantification of histone acetylation was analyzed with Quantity One software (Bio-Rad). (c,d) Mouse lymphocytes were stimulated in vitro and treated with 8f for 36 h. Apoptosis was detected by Annexin V and PI staining, and the percentages of apoptotic cells (Annexin V single positive) were analyzed by flow cytometry.