Figure 3.

Compound 8f alleviates clinical symptoms of EAE. (a,b) Clinical score of EAE mice treated with 8f (n = 10) or PBS (n = 10) b.i.d. by oral administration on the day indicated by the arrow. Data are mean ± SEM, ***p < 0.001 (two-way ANOVA test). (c,d) Representative H&E or Luxol fast blue stained spinal cord sections isolated from naive, 8f, or vehicle-treated EAE mice on day 22 postimmunization. Boxed areas in left columns are presented enlarged on the right. (e,f) Quantitative analysis of the number of spinal cord infiltrates in the sections presented in panel c and the amount of demyelination presented in panel d. Data are mean ± SEM; ***p < 0.001 versus naive group; ^###p < 0.001 versus vehicle control (Student’s t-test).