Figure 2.

Immunogold labeling of axoneme-associated IFT particles. A. Gold labeling is specifically localized on the particles that are associated with the axoneme tip. Arrowheads point to some of the gold particles; arrows indicate microtubular doublets. B. Linear strings of particles interconnected by oblique links are visible at the A tubule tip. The periodicity of the string shown here is about 38 nm, which is close to the one reported for the anterograde trains in situ (40 nm). Immunolabeling in A and B has been obtained with the complex A-specific antibody IFT 139.