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Type I collagen protein expression was determined (a) qualitatively by immunostaining and (b) quantitatively by the hydroxyproline assay on various sIPNs. Osteogenesis was characterized by calcium deposition on the matrix by (c) Alizarin red S staining and (d) quantified using a calcium detection kit on various sIPNs. *p<0.05, ANOVA and Tukey post-hoc tests with n = 3 sIPNs.
