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. 2014 Jun 17;9(6):e99513. doi: 10.1371/journal.pone.0099513

Figure 2. Effect of different concentrations of Cys, Asp, and Glu on S. mutans biofilm formation.

Figure 2

(A) The quantification of S. mutans biofilm formation was evaluated by crystal violet staining after 24 h of challenge with Cys, Asp, and Glu at 40 mM, 20 mM, and 10 mM. The results show the average of three independent experiments repeated five times, and mean and standard deviation were shown. “*” indicates a significant difference in the OD value of S. mutans biofilm between the amino acid challenge groups and the control, and a value of p<0.00833 was considered significant; “#” indicates a significant difference in the OD value of the S. mutans biofilm between the groups challenged with the D- and L-enantiomers of the same amino acid, and a value of p<0.05 was considered significant. (B) S. mutans culture and BHIS at a 1∶100 ratio were added into 24-well plates and challenged with D- or L-Cys, -Asp, and -Glu at 40 mM for 24 h. The S. mutans biofilm formation was visualized by crystal violet staining.