Table 1. Pathotyping and replication of intravenously administrated H7N9/PR8 transfectant virus in chickensa.
Virus | Mortality(death/total) | Virus isolation from swabsb | Seroconversionc(seroconverted/total) | |||
Oropharyngeal | Cloacal | |||||
Shedding/total | Titers | Shedding/total | Titers | |||
PR8 | 0/8 | 1/8 | 1.2±0.08 | 5/8 | 3.2±0.02 | 7/8 |
H7N9/PR8 | 0/8 | 0/8 | ≤1.0 | 0/8 | ≤1.0 | 3/8 |
Groups of chicken were infected i.v. with 0.2 ml of 1∶10 dilution of stock virus.
Oropharyngeal and Cloacal swabs were collected for virus titration on day 3 after inoculation. A mean value of 1.0 was considered for calculation if virus was not isolated from a swab.
Chickens were bled at 2 weeks after inoculation. Sero-conversion was confirmed and analyzed.