Table 2. Summarized table of percentage of positive seedlots with compatible, incompatible and null-interacting bacterial species developed in fruits upon flower inoculation.
1Type of seed health assay | 2Type of interactions with host or non-host plants | ||
Compatible | Incompatible | Null | |
3Plating | 31.7a | 30.9a | 16.8b |
4Symptomatic transmission | 22.4A | 0B | 0B |
5Asymptomatic transmission | 44.6a | 19.5b | 9.4c |
Acidovorax citrulli, C. michiganensis subsp. michiganensis, P. syringae pv. tomato, X. euvesicatoria, P. syringae pv. glycinea and Serratia marcescens were cross-inoculated on to the flowers of watermelon, tomato, pepper, soybean and onion plants. Seeds developed in inoculated fruits were harvested and tested for the presence of target bacteria by plating on semi-selective medium or seedling grow out (SGO) assay.
Type of interaction of bacterial species with their host and non-host plants as determined by foliar infiltration of 1×108 colony forming units/ml.
Mean percentage of seedlots tested positive for compatible, incompatible and null-interacting bacterial species by plating. Means with similar letters are not significantly different according to LSD in SAS (P<0.05).
Mean percentage of seedlots tested positive by symptomatic transmission of compatible, incompatible and null-interacting bacterial species. A seedlot was considered to be positive when a seedling in a tray showed symptoms from any of the three replicates tested. Means with similar letters are not significantly different according to LSD in SAS (P<0.05).
Mean percentage of seedlots tested positive for asymptomatic (epiphytic) transmission of compatible, incompatible and null-interacting bacterial species to developing seedlings. A seedlot was considered to be positive when the target bacterium was recovered from any of the three replicates tested. Means with similar letters are not significantly different according to LSD in SAS (P<0.05).