Figure 6. Biological Validation: overlap with cardiac enhancers.

OccuPeak, MACS and CisGenome were used to call peaks from the TBX3 and the two replicate p300 ChIP-seq datasets. Peaks were then sorted on peak significance and overlap with cardiac enhancers was determined. For visualization, the number of most significant peaks was incremented in steps of 1000 peaks. A set of 102 validated cardiac enhancers was used to assess the sensitivity of the peak-calling method and the biological relevance of the called peaks. The number of enhancers identified using the default threshold of each peak calling method is plotted in the bar graphs.