Table 5. The PCR primers of all exons of DLG1 and PDCL.

Primer ID	F-primer sequences (5′-3′)	R-primer sequences (5′-3′)	Annealing temperature (°C)
DLG1
Exon 1	CCGACTTCTGTCTGTTCTT	GGACCGTGCTGTCTCAT	54
Exon 2	CTCCTCCGTTTTCTAATG	GTTACCGAATGCCTCAG	51.5
Exon 3	GTTAAGTAGTTTGCCTGAACTTGTAGC	CAGATGAAGCCTTGTTGAGGTCT	62
Exon 4	GGTAAGAAATGAGCAATCAATATTCAG	GGGCGAACCTACATGAAAGAATA	55.5
Exon 5	TTTATCTTTATGGCACAGC	AAATGGCAAATCCTGACT	51.5
Exon 6	TTCTGTTTGGTGCTGGAG	GGTCTTCGCATTTGTATC	55.5
Exon 7	CAGAGAAGGATCGGAGGTTGA	GTAAATGGAAACTCTTGGGACTATC	58.8
Exon 8	CCTCCAGAACAAGTCCA	GTATTTATCCCTTATCCAGTC	51.5
Exon 9	TGTTCCTTTTGCTGGCCCTT	ATGACTGCACCACTGGACTC	63
Exon 10	TTCGTAACTCTAGGAGCAGCTGT	CTGTGCATACAAGCCCTCAAC	61
Exon 11	AGACTGGGAGAATAGGAGG	TCACTAATGGCATCACAAC	55.5
Exon 12	TTGAGACTAACCTGGGCAACAT	AAGGACAATTTACCAAGCCTCAA C	61
Exon 13	CTTCTAAGTAGGGGCAGTG	AATAGGTCCAGTGAAAATAAC	54
Exon 14	CAGTAGGCGTGAGAATGTGGC	GCCTGGGCAGTAAGAGTGGA	63
Exon 15	GATTACTGCTGTCTGATGC	GCCTCCTTTGCTACTATG	55.5
Exon 16	TCAATATAACTTACCATTGGATTACAATC	AGTACTATTACCTGTAGTTGCCATGCT	57
Exon 17	TTAAACTCAGAAATGGTGCCTCA	GGTCTGTGAAATGGGTGCTTG	61
Exon 18	AGGTATAAATGAACTATGCTGTCTGAA	CCTTGAAGACAATTAGCAACCTG	58.8
Exon 19	AGTTTGTCCCCTTTGCC	TCAGAATCCCTCCACCC	55.5
Exon 20	AAATAAAGGAGTAGCACATAGC	GAAAGAAGTGGGATAAACAG	54
Exon 21	CATCTTTGGTTGATGGTAGAGTGAG	AGAAAGGACAATAATATGGAGGATG	58.8
Exon 22	ATCCATCCTCCATATTATTGTCCT	ACCCGGCCCTTATCTCCT	57
Exon 23	TTTCATTTCCTATCTAAAGTTTGCTG	ATGGTTCTGCCTCACATTCTGT	57
Exon 24	TGTGTCATCTCTCCTTTGCCA	GAGCCGAGTCATACCATTGC	62
Exon 25	CTATGGGATTGTACCCAGTTTCC	GGTCAGGCCATTCCATCTTC	57
PDCL
Exon 1	TGTCCTGGAAATTGTAGGATCTCA	GACTAGGTTACCTCTGAAAGTGGGA	60.5
Exon 2	ATGTTGGGCATTAGCTTGGC	TTTGACAGGGCTCTATGATTTCTC	60.5
Exon 3-1	TCAAGTGATCCGCTCGTCT	AGCTTCAAGGTCCACAGCA	60.5
Exon 3-2	GCCAGCAGTCAGTTCACCAG	TTTGACAGGGCTCTATGATTTCTC	60.5