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. 2014 Jun 17;9(6):e100016. doi: 10.1371/journal.pone.0100016

Figure 1. MoPrP adsorption into HS affects protein solubility.

Figure 1

SDS-PAGE gel showing the precipitation of full-length MoPrP induced by increasing amounts of HA and FA. After adding 20 and 10 µg/mL of HS (HA and FA, respectively) the total protein is mainly present in the insoluble fractions (A). Decreased MoPrP solubility in the presence of 1 to 5 µg/mL of FA after 6 hours of incubation, and 1 to 10 µg/mL of HA after 6 and 240 hours of incubation (B). Normalized far-UV CD spectra of MoPrP after 6- and 240-hour incubation with 5 µg/mL of HS (C).