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. 2014 Jun 17;9(6):e100266. doi: 10.1371/journal.pone.0100266

Figure 2. TLR agonists promoted RASF to produce inflammatory cytokines.

Figure 2

RASF were stimulated with TLR2 agonist PGN (10 µg/ml), TLR3 agonist ploy(I:C) (25 µg/ml), TLR4 agonist LPS (100 ng/mL), or TLR9 agonist CpG (10 µg/ml). 4 h later, the cells were harvested for RT-PCR (A–C, a) and realtime PCR (A–C, b) analyses of IL-6, IL-8, and TNF-α; 24 h later, the cell culture supernatants were collected for ELISA analyses of IL-6, IL-8, and TNF-α (A–C, c). The results were presented as mean+SEM of five independent experiments (Wilcoxon signed-rank test, *P<0.05 vs. vehicle control).