Skip to main content
. 2014 Jun 17;9(6):e100266. doi: 10.1371/journal.pone.0100266

Figure 3. vLigation of TLRs stimulated MMPs and VEGF production in RASF.

Figure 3

RASF were stimulated with 10 µg/ml PGN, 25 µg/ml ploy(I:C), 100 ng/mL LPS, or 10 µg/ml CpG for 4 h. Then the cells were harvested for RT-PCR analysis of MMP-1 (A); realtime PCR analyses of MMP-1 (B), MMP-3 (C), MMP-9 (E), and VEGF (F). And also, after stimulation for 24 h, the cell culture supernatants were collected for MMP-3 ELISA (D). The results were presented as mean+SEM of five independent experiments (Wilcoxon signed-rank test, *P<0.05 vs. vehicle control).