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. 2014 Jun 17;9(6):e100311. doi: 10.1371/journal.pone.0100311

Table 1. Quantitative PCR primers.

Genes sequences (5′ to 3′) Amplicon
Forward Reverse (bp)
Human ACTB GGCACCCAGCACAATGAAG GCCGATCCACACGGAGTACT 67
TC1 CAAGCCATCATCATGTCCAC GTTGCCCACGGCTTTCTTAC 86
PU.1 GACACGGATCTATACCAACGCC CCGTGAAGTTGTTCTCGGCGAA 145
TREM-1 AAGGAGCCTCACATGCTGTT CACAGTTCTGGGGCTGGTAT 160
Mouse Actb GATCATTGCTCCTCCTGAGC ACATCTGCTGGAAGGTGGAC 83
Ccnd-1 CCCAACAACTTCCTCTCCT TCCAGAAGGGCTTCAATCTG 110
Cebpα TTACAACAGGCCAGGTTT CTCTGGGATGGATCGATT 232
Flt3l CGCTGGATAGAGCAACTGAAGA TGTTGGTCTGGACGAATCGCAG 138
Gata-1 ACTGTGGAGCAACGGCTA TTCCTCGTCTGGATTCCA 301
G-CSF ATGCCGAAGGCTTCCCTG AGGAGACCTTGGTAGAGG 98
GM-CSF AACCTCCTGGATGACATG AAATTGCCCCGTAGACCC 133
Tc1 ACCAGCATGTCCTCGTCTCT ATGTTGCCCACAGCTTTCTT 89
Klf4 CTATGCAGGCTGTGGCAAAACC TTGCGGTAGTGCCTGGTCAGTT 150
M-CSF GCCTCCTGTTCTACAAGT ACTGGCAGTTCCACCTGT 125
Myc AGTGCTGCATGAGGAGACAC GGTTTGCCTCTTCTCCACAG 103
Pou5f1 CAGCAGATCACTCACATCGCC GCCTCATACTCTTCTCGTTGGG 128
Pu.1 TACCAACGTCCAATGCATGA GCTGGGGACAAGGTTTGATA 302
Sox2 AACGGCAGCTACAGCATGATGC CGAGCTGGTCATGGAGTTGTAC 138
Trem-1 TTTCCTCTCCTGGTCTTGGA TCATCCAAATGTCCTCTTTGTG 155