Table 1. Quantitative PCR primers.
Genes | sequences (5′ to 3′) | Amplicon | ||
Forward | Reverse | (bp) | ||
Human | ACTB | GGCACCCAGCACAATGAAG | GCCGATCCACACGGAGTACT | 67 |
TC1 | CAAGCCATCATCATGTCCAC | GTTGCCCACGGCTTTCTTAC | 86 | |
PU.1 | GACACGGATCTATACCAACGCC | CCGTGAAGTTGTTCTCGGCGAA | 145 | |
TREM-1 | AAGGAGCCTCACATGCTGTT | CACAGTTCTGGGGCTGGTAT | 160 | |
Mouse | Actb | GATCATTGCTCCTCCTGAGC | ACATCTGCTGGAAGGTGGAC | 83 |
Ccnd-1 | CCCAACAACTTCCTCTCCT | TCCAGAAGGGCTTCAATCTG | 110 | |
Cebpα | TTACAACAGGCCAGGTTT | CTCTGGGATGGATCGATT | 232 | |
Flt3l | CGCTGGATAGAGCAACTGAAGA | TGTTGGTCTGGACGAATCGCAG | 138 | |
Gata-1 | ACTGTGGAGCAACGGCTA | TTCCTCGTCTGGATTCCA | 301 | |
G-CSF | ATGCCGAAGGCTTCCCTG | AGGAGACCTTGGTAGAGG | 98 | |
GM-CSF | AACCTCCTGGATGACATG | AAATTGCCCCGTAGACCC | 133 | |
Tc1 | ACCAGCATGTCCTCGTCTCT | ATGTTGCCCACAGCTTTCTT | 89 | |
Klf4 | CTATGCAGGCTGTGGCAAAACC | TTGCGGTAGTGCCTGGTCAGTT | 150 | |
M-CSF | GCCTCCTGTTCTACAAGT | ACTGGCAGTTCCACCTGT | 125 | |
Myc | AGTGCTGCATGAGGAGACAC | GGTTTGCCTCTTCTCCACAG | 103 | |
Pou5f1 | CAGCAGATCACTCACATCGCC | GCCTCATACTCTTCTCGTTGGG | 128 | |
Pu.1 | TACCAACGTCCAATGCATGA | GCTGGGGACAAGGTTTGATA | 302 | |
Sox2 | AACGGCAGCTACAGCATGATGC | CGAGCTGGTCATGGAGTTGTAC | 138 | |
Trem-1 | TTTCCTCTCCTGGTCTTGGA | TCATCCAAATGTCCTCTTTGTG | 155 |