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. 2014 Jun 12;7:985–994. doi: 10.2147/OTT.S62887

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© 2014 Martinez-Serra et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Higher levels of the B-CI enable efficient monitoring of cell cytotoxicity.

Notes: Jurkat cells were incubated in the presence of the different substrates (fibronectin, collagen, or gelatin) and FasL. Simultaneously, we performed an MTT (mean ± SEM) assay in order to compare the data obtained with the RTCA with that from a conventional method.

Abbreviations: B-CI, baseline cell index; FasL, Fas ligand; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; RTCA, real-time cell analysis; SEM, standard error of the mean; OD, optical density.