Figure 5.
Effects of SiO2 particles on the expression of Krebs cycle enzymes (IDH and CS) in BRL cells.
Notes: (A) BRL cells were exposed to different concentrations of SiO2 particles for 24 hours. Aliquots of mitochondrial protein were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and analyzed by Western blotting. COX IV was used as an internal control to monitor for equal loading. Densitometry showing data for (B) IDH and (C) CS. The results are the mean ± standard deviation of three independent experiments. *P<0.05 versus the control.
Abbreviations: CS, citrate synthase; IDH, isocitrate dehydrogenase; BRL, buffalo rat liver; COX, cytochrome C oxidase.