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. 2014 Jun 10;8:745–757. doi: 10.2147/DDDT.S62659

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© 2014 Baiula et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Map and Dex eye drops increase, in a dose-related manner, the number of positive apoptotic eosinophils detected by TUNEL staining and chromotrope-2R counterstaining, in tarsal conjunctival sections prepared from guinea pigs sacrificed 24 hours after OVA challenge.

Notes: Ctrls received the vehicle alone and were not treated with topical OVA. Each group comprised five guinea pigs and both eyes were evaluated (n=10). Representative micrographs, in which TUNEL-positive cells appear as distinct, rounded, bright green nuclei and chromotrope-2R-positive cells appear as red spots, are shown. Colocalization of TUNEL and chromotrope-2R staining originates yellow cells (insets show enlargements). Scale bar equals 50 μm.

Abbreviations: Ctrl, control; Dex, dexamethasone; Map, mapracorat; OVA, ovalbumin; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.