Skip to main content
. 2014 Apr 14;8(1):153–158. doi: 10.3892/etm.2014.1684

Figure 5.

Figure 5

Effect of SP600125 in vitro on the expression of claudin-4 and JNK phosphorylation. The cells were treated with different concentrations of SP600125. The cells were then lysed and the protein was extracted in order to determine the expression of (A and B) claudin-4 and (C) p-JNK phosphorylation by western blot analysis using anti-claudin-4, anti-phospho-JNK, anti-JNK and anti-GAPDH antibodies. The expression of each protein was measured using the relative band intensities. *P<0.05 vs. control; #P<0.05 vs. ALI. ALI, acute lung injury; NS, normal saline; p-JNK, phosphorylated c-Jun NH2-terminal kinase.