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Effect of BAY on collagen I and α-SMA expression in vitro. HSC-T6 cells were pretreated with the indicated concentrations of BAY 1 h prior to incubation with 1 μg/ml LPS for 24 h. Collagen I and α-SMA proteins were detected by western blotting and β-actin was used as a internal control. Data were normalized against β-actin. #P<0.01, vs. normal; *P<0.01, vs. LPS-stimulated cells. LPS, lipopolysaccharide; HSC, hepatic stellate cell; BAY, BAY-11–7082; SMA, smooth muscle actin.
