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. 2014 Apr 14;8(1):95–99. doi: 10.3892/etm.2014.1682

Figure 3.

Figure 3

Effect of BAY on the phosphorylation of PI3K/Akt in vitro. HSC-T6 cells were pretreated with various concentrations of BAY for 1 h prior to stimulation with 1 μg/ml LPS for 24 h. Total and phosphorylated PI3K/Akt levels were detected by western blotting and β-actin was used as a loading control. Data were normalized against β-actin expression levels. #P<0.01, vs. normal; *P<0.01, vs. LPS-stimulated cells. LPS, lipopolysaccharide; HSC, hepatic stellate cell; BAY, BAY-11–7082; PI3K, phosphatidylinositol 3-kinase; Akt, protein kinase B.