Figure 4.

Effect of BAY on CCl₄-induced liver fibrosis. Mice were intraperitoneally injected with 1 ml/kg CCl₄ twice a week in combination with 5 mg/kg BAY three times a week for 6 weeks. (A–D) Liver fibrosis was detected by Sirius red staining. Representative micrographs of histology from (A) normal, (B) vehicle (10% DMSO/PBS), (C) CCl₄ and (D) CCl₄ + BAY treated mice (magnification, ×100). (E) Morphometrical analysis for evaluating the percentages of α-SMA-positive areas in 12 random fields. (F) Hepatic hydroxyproline was detected. All data are expressed as the mean ± SD of 12 mice. ^#P<0.01, vs. control; ^*P<0.01, vs. CCl₄. BAY, BAY-11–7082; SMA, smooth muscle actin; CCl₄, carbon tetrachloride; DMSO, dimethyl sulfoxide; PBS, phosphate-buffered saline.