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. 2014 May 19;8(1):219–228. doi: 10.3892/etm.2014.1722

Figure 1.

Figure 1

Effect of TIPE2 on NF-κB p65 protein expression in LPS induced RAW264.7 cells. TIPE2 siRNA and control RAW264.7 cells were incubated with various concentrations of LPS for 9 h, or 10 ng/ml LPS for various time periods. NF-κB p65 protein expression was measured using western blot analysis. Values are expressed as the mean ± standard deviation of three independent experiments. Groups: 1, TIPE2 siRNA RAW264.7 cells; 2, RAW264.7 cells; C, control. (A) Dose and (B) time dependent effects of LPS on NF-κB p65 protein expression in RAW264.7 cells and TIPE2 siRNA RAW264.7 cells. (A) P<0.05, group 1 (5, 10 and 15 mg/ml) vs. group 2 (5, 10 and 15 mg/ml); group 1: *P<0.05, **P<0.01, vs. control; group 2: #P<0.05, ##P<0.01, vs. control. (B) P<0.05, group 1 (6, 9 and 12 h) vs. group 2 (6, 9 and 12 h); group 1: *P<0.05, **P<0.01, vs. control; group 2: #P<0.05, ##P<0.01, vs. control. TIPE2, tumour necrosis factor-α induced protein 8 like 2; NF-κB, nuclear factor κB; LPS, lipopolysaccharide.