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. 2014 May 19;8(1):219–228. doi: 10.3892/etm.2014.1722

Table I.

Primer sequences of the genes used to validate the microarray analysis by qPCR.

Gene Primer Product (bp)
TIPE2 F: 5′-GGGAACATCCAAGGCAAG-3′
R: 5′-AGCTCATCTAGCACCTCACT-3′
195
MIF F: 5′-ATGCCTATGTTCATCGTGAAC-3′
R: 5′-GGCTACGACGAAGGTGGAAC-3′
341
NF-κB F: 5′-GCACGGATGACAGAGGCGTGTATAAGG-3′
R: 5′-GGCGGATGATCTCCTTCTCTCTGTCTG-3′
420
IκB F: 5′-TGCTGAGGCACTTCTGAG-3′
R: 5′-CTGTATCCGGGTGCTTGG-3′
421
β-actin F: 5′-GATTACTGCTCTGGCTCCTGC-3′
R: 5′-GACTCATCGTACTCCTGCTTGC-3′
190

qPCR, quantitative polymerase chain reaction; TIPE2, tumour necrosis factor-α-induced protein 8-like 2; MIF, macrophage migration inhibitory factor; NF-κB, nuclear factor-κB; F, forward; R, reverse.