Table I.
Gene | Primer | Product (bp) |
---|---|---|
TIPE2 | F: 5′-GGGAACATCCAAGGCAAG-3′ R: 5′-AGCTCATCTAGCACCTCACT-3′ |
195 |
MIF | F: 5′-ATGCCTATGTTCATCGTGAAC-3′ R: 5′-GGCTACGACGAAGGTGGAAC-3′ |
341 |
NF-κB | F: 5′-GCACGGATGACAGAGGCGTGTATAAGG-3′ R: 5′-GGCGGATGATCTCCTTCTCTCTGTCTG-3′ |
420 |
IκB | F: 5′-TGCTGAGGCACTTCTGAG-3′ R: 5′-CTGTATCCGGGTGCTTGG-3′ |
421 |
β-actin | F: 5′-GATTACTGCTCTGGCTCCTGC-3′ R: 5′-GACTCATCGTACTCCTGCTTGC-3′ |
190 |
qPCR, quantitative polymerase chain reaction; TIPE2, tumour necrosis factor-α-induced protein 8-like 2; MIF, macrophage migration inhibitory factor; NF-κB, nuclear factor-κB; F, forward; R, reverse.