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Micrographs of hepatocytes following treatment with 1 mmol/l HFFA and increasing doses of CJ for 24 h. Cells were treated with HFFA alone or with CJ extract for 24 h. Cells were stained with Oil Red O to label the fatty acid accumulation. When CJ was incubated with HFFA, Oil Red O staining was reduced. Upper panel: magnification, ×200; lower panel: magnification, ×400. CJ, Cirsium japonicum; HFFA, free fatty acid.
