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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Int J Cancer. 2014 Feb 18;135(5):1238–1246. doi: 10.1002/ijc.28747

Figure 3.

Figure 3

Viral-mediated tumor vascular collapse leads to profound anti-angiogenic changes within the tumor microenvironment. (A) Hypoxic regions in the tumor. Mice (BALB/c mice with 4T1 subcutaneous tumors treated intravenously with 1×108 PFU of vvDD 48h earlier) were injected intravenously with Hypoxyprobe 15 minutes prior to sacrifice. Hypoxic regions were then stained post mortem (red) along with virus (green) and nuclei (Hoescht; blue); (B) Apoptosis within the tumor. Apoptosis was also determined with annexin-V staining (red) on sections post mortem 48h after treatment (C) VEGF levels in the tumor. In an additional experiment mice were sacrificed at the indicated times after vvDD (or PBS) treatment as before. Levels of VEGF within the tumor were determined by Luminex assay (MFI= Mean Fluorescence Intensity; VEGF levels between day 0 and 3; p=0.012).