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Anti-angiogenic effects of oncolytic vaccinia therapy in vitro; (A) Production of VEGF as determined by ELISA in DMEM media alone and from conditioned medium collected from 4T1 or Renca cells alone or 24h after infection (MOI of 1.0 or 0.1) with vvDD; (B) Effects of conditioned medium from uninfected 4T1 cells or infected 4T1 cells (MOI 1.0 and 0.1, and filter sterilized at 0.1 μm to remove any viral particles) on proliferation of HUVEC, as determined by BrdU assay.
