Table 4.
RT-qPCR confirmation of microarray results
| Gene symbol | Microarray fold change | RT-qPCR fold change | |||||
|---|---|---|---|---|---|---|---|
| SYBR® Green | TaqMan® | ||||||
| Min | Max | Mean | FC | P value | FC | P value | |
| AKR1C1 | 2.3 | 2.9 | 2.5 | 1.7 | 0.433 | 2.4 | 0.235 |
| HBB | −2.2 | −2.6 | −2.4 | 0.2 | 0.020 | 0.3 | 0.021 |
| NCAM1 | −1.1 | 2.5 | −0.3 | 0.5 | 0.160 | - | - |
| NR4A2 | 1.1 | −2.1 | −1.6 | 0.4 | 0.248 | - | - |
| USP16 | −1.2 | −5.5 | −2.6 | 0.5 | 0.308 | - | - |
| SAP18 | −1.2 | −2.6 | −1.7 | 0.8 | 0.393 | - | - |
| SERPINA3 | 10.0 | 16.0 | 13.0 | 18.7 | 0.0001 | 15.3 | 0.0005 |
| HBA2 | - | - | - | 0.2 | 0.041 | 0.2 | 0.019 |
| IRF3 | 2.0 | 2.1 | 2.0 | 1.3 | 0.123 | - | - |
| APOC1 | 4.3 | - | 4.3 | 6.3 | 0.047 | 6.8* | 0.028* |
| TTR | 3.1 | - | 3.1 | 7.1 | 0.025 | 5.9 | 0.076 |
Differential expression of selected genes analyzed by microarray and RT-qPCR. For microarray analysis, the lowest (Min), the highest (Max) and the average (Mean) fold change values of all the respective probes are shown. For RT-qPCR analysis, fold change (FC) and statistical significance (p-value) for both SYBR® Green and TaqMan® results are shown. In bold are the genes validated with statistical significance. HBA2 was not present in the array chip.
*Normalization performed vs. ACTB only.