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. 2014 Jun 9;5:3979. doi: 10.1038/ncomms4979

Figure 7. IL1β-eRNA and IL1β-RBT46(+) regulate LPS-induced IL1β and CXCL8 expression and release.

Figure 7

Human monocytic THP-1 cells (Anti-RBT46+) were transfected with two negative control LNAs or an LNA antisense against either IL1β-eRNA (Anti-eRNA) or IL1(β)-RBT46(+) (Anti-RBT46+) at a final concentration of 30 nM. Cells were then treated with either buffer (non-stimulated) or LPS prior to quantification of (a) IL1β-eRNA1 and IL1β-RBT46(+) expression at 2 h (b) IL1β mRNA, CXCL8 mRNA and IL6 mRNA at 24 h, (c) IL1β, CXCL8 and IL6 protein release at 24 h and (d) IL1α mRNA and IL1RN mRNA at 24 h. Data are the mean±s.e.m. of nine independent experiments. Statistical significance was determined using a one-way analysis of variance with a Dunnett’s post test, where *P<0.05, **P<0.01 and ***P<0.001.