Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2014 Jun 18.

Published in final edited form as: Free Radic Biol Med. 2010 Mar 11;48(12):1588–1600. doi: 10.1016/j.freeradbiomed.2010.02.037

Fig. 2 — SM(d18:1/16:0) was modified with HOCl (either added as reagent or generated enzymatically by the MPO–H₂O₂–chloride system) as described under Materials and methods. After modification, lipids were extracted and analyzed by MALDI-TOF-MS using DHB as matrix as described under Materials and methods. Representative spectra of (A) SM(d18:1/16:0), (B) HOCl-modified SM(d18:1/16:0), and (C) MPO-modified SM(d18:1/16:0) are shown. (D) Proposed structures and resulting masses of the chlorinated products.

Fig. 2 — SM(d18:1/16:0) was modified with HOCl (either added as reagent or generated enzymatically by the MPO–H₂O₂–chloride system) as described under Materials and methods. After modification, lipids were extracted and analyzed by MALDI-TOF-MS using DHB as matrix as described under Materials and methods. Representative spectra of (A) SM(d18:1/16:0), (B) HOCl-modified SM(d18:1/16:0), and (C) MPO-modified SM(d18:1/16:0) are shown. (D) Proposed structures and resulting masses of the chlorinated products.