Figure 6.
Effect of (R,S)-norketamine on the levels of phospho-active forms of mTOR, Akt, ERK1/2, p70S6K and 4E-BP1 in PC-12 cells. A, Cells were treated with different concentrations of (R,S)-norketamine (0 – 1 μM) for 1 h and processed for Western blot analysis. (B), Scatter plots illustrating the relative ratio of phosphorylated versus total forms of mTOR, Akt, ERK1/2, p70S6K and 4E-BP1 in response to cell treatment with 25 nM of (R,S)-norketamine are shown (n = 3 independent experiments). *, ** P< 0.05, 0.01 (ANOVA) compared with control cells.