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. Author manuscript; available in PMC: 2014 Jun 18.
Published in final edited form as: J Pathol. 2013 Jun;230(2):194–204. doi: 10.1002/path.4073

Figure 5.

Figure 5

Wnt/β-catenin signalling was inhibited by DACT2. (A) Results of TCF/LEF luciferase reporter assay. Relative luciferase activity (the ratio of firefly luciferase to renilla luciferase) was suppressed by DACT2. Increased luciferase activity was induced by co-transfection of DACT2 with Dvl2. Inhibition of β-catenin/TCF luciferase reporter activity was tempered by co-transfection of β-catenin and DACT2 in a dose-dependent manner. The experiment was repeated three times. (B) Expression of c-Myc and cyclin D1 was inhibited by re-expression of DACT2 in NCI-H23 cells. Expression of c-Myc and cyclin D1 was increased in NCI-H727 cells by knocking down DACT2. Re-expression of DACT2 was found in DACT2-transfected NCI-H23 cells. NCI-H727 cells were the DACT2 expression control.