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Restoration of SOX17 expression inhibited clonogenicity in H23 cells. A. H23 cells transfected with empty control vector or SOX17 expression vector were reseeded at 800 cells/well and selected with 500 µg/ml G418 for 14 days. Cells were observed and counted after fixing with 75% ethanol and staining with 0.2% crystal violet. B. Experiments were repeated in triplicates and the average colony number for each transfected group is expressed by a bar graph.
