Fig. 2.
Schematic representations of high-throughput assays used to generate binary interactome networks. (A) Yeast two-hybrid (Y2H). (B) Protein fragment complementation assays (PCA). (C) Luminescence-based mammalian interactome mapping (LUMIER). (D) Well-based nucleic acid programmable protein array (wNAPPA). (E) Mammalian protein–protein interaction trap (MAPPIT). (F) A high-quality reference human binary interactome comprising B40 000 interactions generated from several large-scale interactome mapping efforts and thousands of small-scale studies.