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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: J Mol Recognit. 2013 Aug;26(8):376–381. doi: 10.1002/jmr.2284

Figure 4.

Figure 4

Comparison of S9.6 scFv affinities under several conditions obtained via SPR. (A) Binding between S9.6 scFv and nucleic acid hybrids was measured in ionic strength variations of 1x HBS-T buffer, in the presence of Mg/Ca, in Tris-Mg, and 1x PBS-T. The leftmost lane shows the binding in the standard 1x HBS-T buffer. Note that the RNA-RNA hybrid (23AURR) displayed lower affinity binding under every condition when compared to the DNA-RNA hybrids. (B) Binding interactions were tested in individual experiments with standard 1x HBS-T prepared at different levels of pH. Each point represents the average of three measurements. Error bars denote standard deviation.